Agarose for Gel Electrophoresis

Reuzeit offers several types of agarose: basic, molecular biology grade, low melt, and PFGE grade. Basic agarose is suitable for general use, molecular biology grade is high-purity and suitable for sensitive applications, low melt agarose is ideal for DNA recovery and analysis, and PFGE grade is specialized for separating large DNA fragments. Choose based on the application requirements for your experiment.

The concentration depends on the size of the DNA or RNA fragments you need to separate. Higher agarose concentrations (1.5-2%) resolve smaller fragments, while lower concentrations (0.5-1%) are better for larger fragments. Check your experiment’s requirements to select the optimal concentration.

This agarose is primarily designed for DNA and RNA separation in electrophoresis. For protein separation, polyacrylamide gels are generally more suitable.

Low melt agarose is useful for applications where DNA needs to be recovered from the gel, as it allows easy gel melting at lower temperatures without damaging the DNA. This is ideal for cloning or further analysis.

Agarose should be stored in a cool, dry place away from moisture. Proper storage helps maintain its quality and prevents clumping or degradation.

Generally, agarose gels are not reusable for precise or quantitative work, as they can lose resolution after use. However, for practice or visual reference, they may be reused if the DNA or stain can be removed and the gel remains intact.