Bio-Rad QX200

The Bio-Rad QX200 system is designed for highly accurate detection and quantification of nucleic acids. It is mainly used in clinical diagnostics to aid in detecting and quantifying DNA and RNA, supporting medical decisions for conditions like cancer, viral infections, and transplant rejection.

The QX200 system partitions samples into thousands of nanoliter-sized droplets, each undergoing PCR individually. By analyzing each droplet for PCR-positive or PCR-negative status, the system provides absolute quantification of target DNA, minimizing variability and ensuring precise results.

Yes, the Bio-Rad QX200 is CE-IVD marked, indicating it is approved for in vitro diagnostics in the European Union, making it suitable and validated for clinical laboratory use.

The Bio-Rad QX200 system includes three key components: the QX200 Droplet Generator, the QX200 Droplet Reader, and the QuantaSoft software. Each of these components is CE-IVD marked for clinical applications.

Yes, the Bio-Rad QX200 system is capable of quantifying both DNA and RNA, making it versatile for a range of diagnostic applications in clinical settings.

The QX200 Droplet Generator takes the PCR reaction mixture and partitions it into thousands of tiny droplets, each acting as an individual reaction vessel. This partitioning is essential for achieving the high sensitivity and precision of the digital PCR process.

The system includes QuantaSoft software, which is used to analyze the data from each droplet. It provides the absolute quantification data required for accurate interpretation of the PCR results in clinical diagnostics.

Digital PCR technology provides absolute quantification, meaning it directly counts target molecules rather than estimating them, which improves accuracy and sensitivity. This high precision is particularly beneficial in detecting low-level nucleic acids in challenging clinical samples.

While the system is designed for clinical use, users may benefit from training to fully understand the ddPCR process and software analysis. Bio-Rad typically offers resources or training to help laboratory staff get the most out of the system.

The Bio-Rad QX200 system is intended to function as a standalone digital PCR solution for precise nucleic acid quantification. However, data output can often be integrated into laboratory information management systems (LIMS) depending on lab setup.