Chromatography Media

Chromatography media is used for separating and purifying compounds in laboratory processes. It supports various applications like protein purification, DNA/RNA separation, and small molecule analysis in research, development, and quality control.

There are several types, including silica-based media, polymer-based media, and resin-based media, designed for different separation techniques like ion exchange, affinity, hydrophobic interaction, and size exclusion.

The choice depends on factors like the type of sample, separation goals, compatibility with your equipment, and the specific technique (e.g., HPLC, FPLC, or preparative chromatography).

Some types are reusable depending on the material, the nature of the sample, and the cleaning protocols. Proper maintenance and storage are essential to extend the media's life.

New media offers optimal performance without prior use, used media may have been employed in previous experiments but can still perform adequately, and refurbished media has been cleaned and restored to meet quality standards.

Yes, chromatography media must be stored in a controlled environment, typically at recommended temperatures and in appropriate buffers or solutions to maintain its stability and effectiveness.

Many types are technique-specific, but some versatile media can be adapted for multiple chromatography methods. Verify compatibility with your method before purchasing.

Shelf life varies by type and brand. Manufacturers usually provide a recommended expiry period, which depends on storage conditions and the material's stability.

Check the specifications of your chromatography system and consult with the media's technical datasheet or supplier to confirm compatibility.

Factors like particle size, pore size, surface chemistry, flow rate, and column packing directly influence separation efficiency and resolution. Match these factors to your application requirements for optimal performance.