What is an FPLC system used for?
FPLC systems are used for high-precision protein purification and other biomolecular separations, making them essential for research in biochemistry, molecular biology, and related fields.
How does an FPLC system differ from other chromatography systems?
FPLC systems are specifically designed for biomolecular separations and use low-pressure liquid chromatography techniques, ensuring precise separation of proteins and other sensitive biomolecules.
What components are typically included in an FPLC system?
An FPLC system usually includes a pump, UV detector, fraction collector, control software, and columns specifically designed for biomolecular separations.
Can FPLC systems be customized for specific applications?
Yes, FPLC systems often allow customization by using specific columns, buffers, and settings tailored to the type of biomolecule being separated.
What factors should be considered when selecting an FPLC system?
Key factors include flow rate, pressure range, compatibility with your existing lab setup, and the types of biomolecules you intend to purify.
Is specialized training required to operate an FPLC system?
While FPLC systems are designed to be user-friendly, some basic training or knowledge of chromatography principles is recommended for optimal operation.
How do you maintain an FPLC system for long-term use?
Regular maintenance includes cleaning and calibrating the system, replacing worn components like pump seals, and ensuring proper storage of columns.
What types of samples can be processed with an FPLC system?
FPLC systems are ideal for purifying proteins, peptides, nucleic acids, and other biomolecules from complex mixtures.
Are FPLC systems compatible with a variety of columns?
Yes, FPLC systems are compatible with many column types, including size exclusion, ion exchange, and affinity columns, depending on the separation requirements.
What are the benefits of using refurbished FPLC systems?
Refurbished systems offer cost savings while still providing reliable performance, especially when sourced from trusted vendors who test and validate the equipment.