What types of sequencing applications can I perform with the Illumina Sequencer?
The Illumina Sequencer is versatile and can be used for a wide range of sequencing applications including whole genome sequencing, RNA sequencing, exome sequencing, targeted sequencing, and metagenomics. It is suitable for both small-scale and large-scale sequencing projects.
What is the difference between the HiSeq 2500 and HiSeq 1500 in terms of data output?
The HiSeq 2500 can produce up to 600 Gb of data in a high output run or up to 120 Gb in rapid run mode. The HiSeq 1500 can generate up to 300 Gb in high output mode or up to 60 Gb in rapid run mode. The HiSeq 2500 offers greater data output capacity compared to the HiSeq 1500.
How long does a sequencing run take on the Illumina HiSeq systems?
The run time depends on the mode selected. In high output mode, sequencing can take from 7 to 10 days for larger datasets, while in rapid run mode, it can take as little as 1-2 days, allowing faster turnaround for smaller projects.
Can I adjust the sequencing output based on the size of my project?
Yes, the Illumina Sequencer allows you to switch between high output mode and rapid run mode. This flexibility enables you to tailor the output based on your study's needs, whether you're working with large or small datasets.
What is the maximum number of samples that can be sequenced in a single run?
The number of samples that can be sequenced in a single run depends on the sequencing type and the specific settings, but typically, the Illumina Sequencer can handle a large number of samples, particularly in high output mode. For precise sample capacity, it is recommended to consult the platform's specific protocols based on your sequencing configuration.
What is the sequencing throughput of the Illumina HiSeq 2500 and HiSeq 1500?
The HiSeq 2500 offers a throughput of up to 600 Gb per run in high output mode and up to 120 Gb in rapid run mode. The HiSeq 1500 provides up to 300 Gb in high output mode and up to 60 Gb in rapid run mode.
Is the Illumina Sequencer suitable for high-throughput sequencing projects?
Yes, the Illumina Sequencer is ideal for high-throughput sequencing. Its high output mode supports large-scale studies, enabling sequencing of entire genomes, transcriptomes, or targeted regions with a high level of accuracy.
How flexible is the Illumina Sequencer in terms of sequencing speed and data output?
The Illumina Sequencer offers excellent flexibility with its ability to switch between high output and rapid run modes. This allows you to balance speed with data output depending on the needs of your project, whether you need large amounts of data or faster results.
Can the Illumina Sequencer be used for both small and large sequencing projects?
Yes, the Illumina Sequencer is designed to handle both small and large sequencing projects. By switching between high output and rapid run modes, it can accommodate a wide range of project sizes while maintaining cost-efficiency.
What are the advantages of using the Illumina Sequencer over other sequencing platforms?
The Illumina Sequencer is known for its high accuracy, flexibility, and scalability. Its ability to switch between high output and rapid run modes allows you to optimize both the speed and data output depending on your project needs. Additionally, Illumina’s technology ensures high-quality data, making it a preferred choice for researchers across various fields.