Applied Biosystems StepOne Plus Real-Time PCR System

Product Information

The Applied Biosystems StepOne Plus Real-Time PCR System is a powerful 96-well Real-Time PCR instrument designed to deliver precise, quantitative results for a variety of genomic research applications. This robust LED-based 4-color optical recording system is ideal for first-time and experienced users alike. Whether used for gene expression, genotyping, protein analysis, or microbial quantification, the StepOne Plus provides the dynamic range, sensitivity, and speed required for a wide range of DNA amplification and detection techniques. This molecular biology marvel is a game-changer.

• LED based 4-color optical recording
• High throughput compatibility for multiplexing
• Linear Dynamic Range greater than 9 log units (detection)
• Sustainable packaging with a focus on green features
• Peltier-based thermal cycling system for precise temperature control

• Calibrated Dyes: FAM, JOE, NED, ROX, SYBR Green I, TAMRA, VIC
• Capacity: 1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
• Detection Method: Primer-Probe Detection, SYBR
• Dimensions: 24.6 cm (W) x 42.7 cm (D) x 51.2 cm (H) / 9.7 in. (W) x 16.8 in. (D) x 20.2 in. (H)
• Dynamic Range: Linear Dynamic Range greater than 9 log units (detection)
• For Use With (Equipment): StepOnePlus
• Format: 0.1 ml tubes, 8-tube strips, 96-well plate
• Optics: 4 emission filters, Blue LED excitation source, photodiode
• Peak Block Ramp Rate: 4.6C/sec
• Reaction Speed: Fast, Standard
• Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
• Run Time: <2 hrs run (Standard Mode), <40 min run (Fast Mode)
• Sample Ramp Rate: Fast mode: 2.2C/sec, Standard mode: 1.6C/sec
• Sensitivity: 1 copy
• Temperature Accuracy: 0.25°C (35 to 95°C) of display temperature
• Temperature Range: 4-100°C
• Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature

Metric: 47.0 kg
Imperial: 103.61714 lbs

Metric: 42.7 cm x 24.6 cm x 51.2 cm
Imperial: 16.8 in x 9.7 in x 20.2 in

902780

This code applies to instruments and apparatus for physical or chemical analysis, and used extensively in medical research or for medical diagnosis.