What is a UHPLC Column used for?
A UHPLC Column is used for high-performance liquid chromatography to separate, identify, and analyze complex samples with high precision. It is ideal for applications such as glycan analysis and other advanced analytical tasks.
How does a UHPLC Column differ from an HPLC Column?
UHPLC Columns operate at higher pressures than traditional HPLC Columns, allowing for faster analysis, higher resolution, and better separation of compounds. This results in improved efficiency and accuracy in analytical applications.
Is this UHPLC Column compatible with my existing UHPLC system?
Most UHPLC Columns are designed to be compatible with standard UHPLC systems. However, it's important to check compatibility with your system’s pressure rating, fittings, and detector requirements.
What types of samples can be analyzed using this UHPLC Column?
This UHPLC Column is suitable for complex sample analysis, including biomolecules such as glycans, peptides, proteins, and small molecules in pharmaceutical, biochemical, and environmental research.
What detection methods can be used with this UHPLC Column?
It is compatible with multiple detection methods, including fluorescence and mass spectrometry (MS), ensuring flexibility for different analytical applications.
What are the benefits of using a UHPLC Column?
Using a UHPLC Column provides higher resolution, faster analysis time, improved sensitivity, and greater efficiency in separating complex mixtures compared to conventional HPLC Columns.
How do I maintain the performance of the UHPLC Column?
Proper maintenance includes using the recommended mobile phase, avoiding sample contamination, regularly flushing the column with appropriate solvents, and storing it under specified conditions to prolong its lifespan.
What type of mobile phase should be used with this UHPLC Column?
The choice of mobile phase depends on the application and sample type. It’s important to use high-purity solvents and follow the manufacturer’s recommendations for optimal performance.
Can this UHPLC Column be used for high-throughput applications?
Yes, this column is designed for high-throughput environments, providing fast and reliable results while maintaining superior resolution and selectivity.
How long does a UHPLC Column last?
The lifespan of a UHPLC Column depends on factors such as sample cleanliness, solvent quality, and usage conditions. Proper maintenance and care can extend its longevity significantly.