Invitrogen AFC2 Autosampler Attune NXT, Acoustic Focusing Cytometer System

Invitrogen CytKick MAX Autosampler

**Unit has been tested to specifications. Has slight cosmetic damages for regular use. Bottles not included. See photos for further details. 

Acoustic-assisted hydrodynamic focusing, the Attune NxT Flow Cytometer (Figure 1) avoids compromise between data quality and higher sample rates by uncoupling cell alignment from sheath flow. Acoustic-assisted hydrodynamic focusing precisely aligns cells using ultrasonic radiation pressure (>2 MHz) to transport particles into the center of the sample stream. This prefocused stream is then injected into the sheath stream. Acoustic-assisted hydrodynamic fluidics increase sample input speed while maintaining data integrity. Flat-top lasers deliver more even application of light to each cell.  Invitrogen Attune NxT Software designed to guide users through complex flow cytometry experiments. Fluid storage designed for minimal waste. Volumetric fluidics provides cell counting and a resistance to clogging. Autosampler provides easy 1-click transition from tube to plate. 

The CytKick Max Autosampler is for use with the Attune NxT Acoustic Focusing Cytometer. It enables rapid processing of multiple samples by automatically analyzing samples from 96- and 384-well plates (standard and deep), as well as some custom plate types and microfuge tube racks. The CytKick Max Autosample features a boost mode that enables faster sample processing, as well as passive cooling for some plates/racks. A CytKick Max Accessory Kit is included that contains four accessory plates: two cooling plates and two tube adaptors, as well as a Max dongle key. A computer is not included with the autosampler since one is provided with the cytometer.

The CytKick Max Autosampler has two options for processing samples in a high-throughput environment: high-throughput mode and boost mode. High-throughput mode enables plate screening by collecting 20 µL of sample at a rate of 1000 µL/min, while Boost mode shortens the time between samples and allows the user to select the sample volume and sample input rate.

• Reliable: intelligent probe design minimizes clogging and carryover (0.5%) and prevents damage to the instrument
•  Mixing by aspiration: mixing of sample by aspiration instead of shaking ensures homogeneity of the sample and maintains cell viability
• Easy transition: no disassembly, no additional QC, no reboot required for conversion between plates and tubes
• Boost mode: reduces processing time from 42 min to 22 min for 96-well plate (using one rinse and one mix and full analysis of a 20-µL sample)
• Sample cooling: passive cooling available for 96-well U-bottom plates and microfuge tube.
• Maximum compatibility: 96- and 384-well plates (standard and deep), 96- and 384-well standard-depth foil-covered plates, some custom plate types, and 1.5-mL and 2-mL microfuge tube racks (up to 24 per rack)
• Auto-cleaning: performs automated cleaning when the instrument is shutting down.
• Reliable: The CytKick Max Autosampler was designed to minimize system down time. Automated sampling devices are often plagued by clogging or probe crashes. The Intelligent Probe, with a 200-micron diameter that is larger than most probes available in the industry, minimizes instances of clogging. 
• Sample cooling: The CytKick Max Autosampler is compatible with a 96-well standard-plate universal cooling block that will keep samples at 2-8°C for ∼60 minutes, a 96-well standard-plate extended cooling block that will keep samples at 2-8°C for ∼100 minutes, and a microfuge-tube cooling rack that will keep samples at 2-8°C for ∼100 minutes. The user will need to chill the cooling block/rack in the refrigerator overnight prior to use.
• Maximum compatibility: The CytKick Max Autosampler allows use of many different plate formats, including 96-, 384-, and deep-well plates, without being restricted to a single manufacturer. The CytKick Max Autosampler also enables 1.5- and 2.0-mL microfuge tubes to be run in an automated format.
• Mixing by aspiration: Unlike other plate loaders that mix samples by shaking, which may not ensure homogeneity of the sample, the CytKick Max Autosampler mixes samples by aspiration and stirring, enabling uniform mixing while preserving sample viability and resulting in consistent data with CVs less than 10% across the plate. 
• Improve lab safety with reduced sample handling with
  no-wash protocol. Completely cut out time-consuming centrifugation steps. Save countless hours running dilute samples and reduce reagent costs. Eliminate cell loss due to wash steps or RBC removal procedure. Ideal for limited sample volumes and for functional
  live-cell assays 

Specifications