Applied Biosystems StepOne Plus Real-Time PCR System #4376592

Product Information

The Applied Biosystems StepOne Plus Real-Time PCR System #4376592 is a powerful and highly efficient 96-well Real-Time PCR instrument suitable for both first-time and experienced users. Incorporating robust LED based 4-color optical recording, the StepOne Plus is engineered to produce accurate, quantitative Real-Time PCR results for a wide range of genomic research applications. This versatile system boasts the ability to perform multiplexing, making it ideal for high throughput molecular biology experiments. An array of emission filters, a blue LED excitation source, and a photodiode enable a fast reaction speed and a sample ramp rate, ensuring the system is capable of handling experiments with minimum run times. With a temperature uniformity of 0.25°C (35 to 95°C) and a wide temperature range of 4-100°C, the StepOne Plus provides consistently reliable results across various experimental conditions, even with a Linear Dynamic Range greater than 9 log units. Whether used in standard or fast mode, this instrument offers excellent temperature accuracy, ensuring precision and repeatability in results. The StepOne Plus Real-Time PCR System is suitable for performing standard curve experiments with a reaction volume range of 10-30 l (40 l in standard mode) and is capable of sensitivity to 1 copy. This powerful equipment is designed to meet the demands of modern molecular biology research applications.

• LED based 4-color optical recording
• Sustainable packaging
• Linear Dynamic Range greater than 9 log units
• Multiplexing capability
• Temperature accuracy of 0.25°C (35 to 95°C)
• Reaction volume range of 10-30 l

• Calibrated Dye: FAM, JOE, NED, ROX, SYBR Green I, TAMRA, VIC
• Capacity: 1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
• Detection Method: Primer-Probe Detection, SYBR
• Dynamic Range: Linear Dynamic Range greater than 9 log units
• For Use With (Equipment): StepOnePlus
• Format: 0.1 ml tubes, 8-tube strips, 96-well plate
• Green Features: Sustainable packaging
• High Throughput Compatibility: Multiplexing
• Optics: 4 emission filters, Blue LED excitation source, photodiode
• Passive Reference Dye: No ROX, ROX (Pre-mixed), ROX (Separate Tube)
• Peak Block Ramp Rate: 4.6Csec
• Product Line: StepOnePlus
• Product Size: 1 instrument
• Reaction Speed: Fast, Standard
• Reaction Volume Range: 10-30 l (Standard curve experiments: 40 l in standard mode is validated)
• Run Time: <2 hrs (Standard Mode), <40 min (Fast Mode)
• Sample Ramp Rate: Fast mode: 2.2Csec, Standard mode: 1.6Csec
• Sensitivity: 1 copy
• Temperature Accuracy: 0.25C (35 to 95C) of display temperature
• Temperature Range (Metric): 4-100C
• Temperature Uniformity: 0.25C (35 to 95C) of setpoint/display temperature

Metric: 30.0 kg
Imperial: 66.14 lbs

Metric: 24.02 cm x 15.35 cm x 30.31 cm
Imperial: 9.46 in x 6.05 in x 11.96 in

90279020

9027.90.20 - Other instruments and apparatus for physical or chemical analysis