Digital PCR

Digital PCR (dPCR) is an advanced method for accurately quantifying DNA and RNA. Unlike traditional PCR, which amplifies DNA samples to detect the presence or absence of a target, dPCR quantifies the exact number of DNA or RNA molecules in a sample by partitioning the sample into thousands of smaller reactions. This allows for more precise and sensitive measurements, especially in detecting low-abundance targets or rare mutations.

Digital PCR is widely used in genetic analysis, including mutation detection, gene expression profiling, and biomarker discovery. It is particularly useful in research areas such as cancer genomics, infectious disease detection, and precision medicine, where precise quantification of nucleic acids is essential.

Digital PCR provides superior sensitivity, accuracy, and precision compared to traditional qPCR, especially when quantifying low-abundance targets or rare genetic variants. It does not require standard curves for quantification, which reduces the potential for errors and provides more reliable results in challenging applications.

Yes, one of the key advantages of Digital PCR is its ability to detect rare genetic mutations at very low frequencies. By partitioning the sample into many individual reactions, dPCR can accurately quantify even a small number of target molecules in the presence of a large excess of non-target DNA, making it ideal for rare mutation detection.

The right Digital PCR system depends on the specific requirements of your research, such as the level of sensitivity, throughput, and budget. Factors to consider include the type of samples you work with, the volume of samples to be processed, and whether you require additional features like multiplexing capabilities or high-speed processing.

Yes, Digital PCR can be used for both DNA and RNA quantification. For RNA analysis, a reverse transcription step is typically required to convert RNA into complementary DNA (cDNA) before quantification using dPCR. This makes it a versatile tool for a range of molecular biology applications.

Digital PCR is highly accurate due to its ability to partition samples into many individual reactions, each capable of detecting single DNA or RNA molecules. This method minimizes errors due to amplification efficiency variations and provides precise quantification, even in complex samples.

Refurbished Digital PCR systems offer a cost-effective solution while maintaining high-quality performance. These systems are carefully inspected, tested, and restored to near-new condition by experienced technicians, ensuring they meet the same operational standards as new equipment at a fraction of the cost.

Yes, Digital PCR is excellent for gene expression analysis. It allows for precise quantification of gene expression levels by measuring the number of target mRNA molecules present in a sample. This high level of accuracy is particularly valuable for detecting subtle changes in gene expression.

Digital PCR offers unparalleled sensitivity and precision for biomarker detection, even at very low concentrations. It can accurately detect rare biomarkers in complex samples, making it an invaluable tool in early disease detection, monitoring disease progression, and evaluating treatment efficacy.