Thermo Scientific Varioskan LUX Spectrophotometer Multimode Microplate Reader

Thermo Scientific Varioskan LUX Spectrophotometer (VL0000D0), manufactured in a pre-owned laboratory setting, is an advanced multimode microplate reader for absorbance, fluorescence, and luminescence workflows. The unit powers on and initializes with a solid green indicator light. It supports top and bottom reading and is designed for assays across a wide range of plate formats (6 to 1536 wells), including endpoint, kinetic, spectra, multipoint, and kinetic spectra. Key performance capabilities include monochromator-based wavelength selection for absorbance and fluorescence, spectral scanning for luminescence modes, and automated dynamic range selection to help manage low and high signal intensity in a single run. The system also includes an integrated gas module for O2 and CO2 measurement in the chamber. Full functional qualification could not be completed because compatible software was not available during intake; please validate fitness for your specific application.

Preowned Thermo Varioskan LUX Spectrophotometer from a working laboratory environment. Unit powers on with a solid green indicator light and successfully initializes upon startup. Unable to complete full functional testing due to lack of compatible software. See photos for additional condition details.

• Multimode detection for absorbance, fluorescence, and luminescence
• Top and bottom reading for flexible plate measurement
• Automatic dynamic range selection to adapt measurement range per well
• Smart safety controls to help identify potential issues before measurement
• Monochromator-based wavelength selection for absorbance and fluorescence
• Spectral scanning luminescence modes for wavelength flexibility
• Supports endpoint, kinetic, spectra, multipoint, and kinetic spectra
• Integrated gas module for O2 and CO2 measurement in the chamber
• Optional modular hardware and SkanIt software integration for expanded capabilities

• Voltage: 100-240V
• Hertz: 50Hz/60Hz
• Plate types: 6-384 well plates (absorbance)
• Wavelength selection: Double monochromators (absorbance)
• Wavelength range: 200-1,000 nm (absorbance)
• Light source: Xenon flash lamp (absorbance)
• Linear measurement range: 0-4 Abs at 450 nm, +/- 2% (96-well plate)
• Linear measurement range: 0-3 Abs at 450 nm, +/- 2% (384-well plate)
• Accuracy: 0.003 Abs or +/- 2% at 200-399 nm (0-2 Abs)
• Accuracy: 0.003 Abs or +/- 1% at 400-1,000 nm (0-3 Abs)
• Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0-3 Abs)
• Plate types: 6-1536 well plates (top reading) and 6-384 well plates (bottom reading) (fluorescence)
• Excitation wavelength range: 200-822 nm (fluorescence)
• Emission wavelength range: 270-840 nm (fluorescence)
• Light source: Xenon flash lamp (fluorescence)
• Sensitivity: Top reading <0.4 fmol fluorescein/well (black 384-well plate)
• Sensitivity: Bottom reading <4 fmol fluorescein/well (clear bottom black 384 square well plate)
• Dynamic range: Top reading >6 decades (fluorescence)
• Dynamic range: Bottom reading >5.5 decades (fluorescence)
• Plate types: 6-1,536 well plates (time-resolved fluorescence)
• Excitation wavelength range: Fixed to 334 nm (spectral scanning 200-840 nm) (time-resolved fluorescence)
• Emission wavelength range: 400-670 nm (spectral scanning 270-840 nm) (time-resolved fluorescence)
• Sensitivity: <1 amol Eu/well (white low volume 384 well plate) (time-resolved fluorescence)
• Dynamic range: >6 decades (time-resolved fluorescence)
• Plate types: 6-1536 well plates (luminescence)
• Wavelength range: 360-670 nm (luminescence)
• Sensitivity: <7 amol ATP/well (white 384-well plate) (luminescence)
• Dynamic range: >7 decades (luminescence)
• Plate types: 6-1536 well plates (AlphaScreen)
• Excitation wavelength range: Fixed to 680 nm (AlphaScreen)
• Emission wavelength range: 400-660 nm (AlphaScreen)
• Light source: LED (AlphaScreen)
• Sensitivity: <100 amol phosphotyrosine/well (white 384-well plate) (AlphaScreen)
• Dispensing plate types: 6-384 well plates
• Number of dispensers: None or two
• Syringe size: 1 mL
• Dispensing volume: 2-5,000 uL in 1 uL increments
• Accuracy: <1 uL with 50 uL
• Precision: <1 uL with 50 uL
• Dead volume: Reagent loss <100 uL; total tubing volume <800 uL
• Temperature range: From ambient + 4 C to 45 C (incubator and shaker)
• Shaking type: Orbital
• Measurement chamber O2 concentration accuracy: +/-1.0% (37 C, 1% O2)
• Measurement chamber CO2 concentration accuracy: +/-1.0% (37 C, 5% CO2)
• Measurement chamber O2 concentration: 1-21%
• Measurement chamber CO2 concentration: 0.1-15%
• Gas concentration recovery time: 10 minutes
• Measurement modes: Endpoint, kinetic, spectra, multipoint and kinetic spectra
• Measurement speed: 96-well plate in 15 s (minimum time)
• Measurement speed: 384-well plate in 45 s (minimum time)
• Measurement speed: 1,536-well plate in 135 s (minimum time)
• Interface: PC software (SkanIt software)
• Dimensions: 58 x 53 x 51 cm (D x W x H)
• Actual weight: 117 kg

Imperial: 140.4 Pounds
Metric: 63.68 Kilograms

Imperial: 27.6 in x 24.0 in x 24.0 in
Metric: 70.1 cm x 60.96 cm x 60.96 cm