This column chromatography system automates the separation and purification of semi-preparative compounds, typically proteins, using FPLC Columns. Operating at 4°C to preserve biological activity, it features gradient elutions with two pumps and an automated fraction collector. Choose from five columns: Superdex 75, Superose 12, Mono Q, Mono S, and phenyl sepharose. Ideal for precise and efficient protein purification.
FPLC Columns
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Amersham Biosciences AKTA explorer 100 FPLC Liquid Chromatography System w/ Frac-950
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Question & Answers For:
FPLC Columns
Past Questions, Helpful Answers.
q.
What are FPLC columns used for?
a.
FPLC columns are used for the separation and purification of biomolecules, such as proteins, peptides, and nucleic acids, using fast protein liquid chromatography.
q.
What types of columns are available with this system?
a.
The system includes five column options: Superdex 75, Superose 12, Mono Q, Mono S, and phenyl sepharose, each designed for specific applications like size exclusion, ion exchange, or hydrophobic interaction chromatography.
q.
Can this system maintain low temperatures during operation?
a.
Yes, it operates at 4°C to ensure the biological activity of sensitive samples is preserved.
q.
Is the system compatible with gradient elution methods?
a.
Yes, the system supports gradient elutions using two pumps for precise separation processes.
q.
Does the system include an automated fraction collector?
a.
Yes, it comes with an automated fraction collector to efficiently collect separated components during the purification process.
q.
Are these columns reusable?
a.
Yes, the columns are reusable, but their lifespan depends on the type of sample and cleaning protocols followed.
q.
What is the maximum sample capacity of the columns?
a.
The sample capacity varies by column type, depending on the specific resin and intended use, but typically supports semi-preparative scale processes.
q.
Are special skills required to operate this system?
a.
The system is designed for user-friendly operation and includes automation for gradient elutions and fraction collection. Basic knowledge of chromatography and protein purification is helpful.
q.
What maintenance is required for the columns?
a.
Regular cleaning and storage in recommended buffers are essential to maintain column performance and longevity.
q.
Can this system handle high-pressure applications?
a.
Yes, the system is built to handle pressures typical for FPLC, ensuring optimal performance during semi-preparative purification.