UHPLC System

A UHPLC (Ultra-High Performance Liquid Chromatography) system is used for separating, identifying, and quantifying components in complex mixtures. It is commonly used in pharmaceutical, environmental, chemical, and food industries for high-precision analysis.

UHPLC operates at higher pressures than traditional HPLC, allowing for faster analysis, higher resolution, and better sensitivity. It uses smaller particle sizes in the column, which enhances separation efficiency.

UHPLC systems can analyze a wide range of samples, including pharmaceuticals, biological fluids, food and beverages, environmental samples, and chemical compounds.

UHPLC systems offer higher resolution, faster run times, improved sensitivity, reduced solvent consumption, and better reproducibility compared to conventional HPLC systems.

Key factors include pressure capacity, flow rate, column compatibility, detector options, and software features for data analysis and method development.

No, UHPLC requires specialized columns with smaller particle sizes (typically sub-2-micron) to withstand higher pressures and achieve optimal performance.

Regular maintenance includes flushing the system, replacing filters, checking pump seals, cleaning the autosampler, and ensuring proper calibration to maintain performance and longevity.

Yes, UHPLC is suitable for both qualitative and quantitative analysis, making it ideal for identifying compounds and measuring their concentrations with high accuracy.

Common detectors include UV-Vis, PDA (Photodiode Array), fluorescence, and mass spectrometry (MS) detectors, depending on the application and sensitivity requirements.