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Question & Answers For:

HPLC Autosamplers

Past Questions, Helpful Answers.

q.

What is the purpose of an HPLC autosampler?

a.

An HPLC autosampler automates the process of injecting samples into an HPLC system, ensuring precision and reproducibility while saving time in high-throughput laboratory workflows.

q.

Can HPLC autosamplers handle multiple sample types?

a.

Yes, most HPLC autosamplers are designed to handle a wide range of sample types, including liquids of varying viscosities and sometimes even gaseous or volatile samples, depending on the model.

q.

How does an autosampler improve the efficiency of HPLC analysis?

a.

Autosamplers allow for unattended operation by automatically injecting samples at programmed intervals, reducing human error, and enabling continuous operation, which increases the throughput and reliability of results.

q.

Are HPLC autosamplers compatible with all HPLC systems?

a.

Compatibility depends on the brand and model. It is essential to check whether the autosampler supports your specific HPLC system or is adaptable through accessories or software integration.

q.

What maintenance is required for an HPLC autosampler?

a.

Routine maintenance typically includes cleaning sample trays, checking injection needles or ports for clogs, ensuring the proper functioning of syringes, and calibrating the injection system periodically.

q.

Can autosamplers handle temperature-sensitive samples?

a.

Many HPLC autosamplers include temperature control features, such as cooling or heating, to maintain the stability of temperature-sensitive samples during analysis.

q.

How do I determine the sample volume range of an HPLC autosampler?

a.

Sample volume ranges are specified in the technical documentation of the autosampler. Ensure the model you select supports the volume requirements of your experiments.

q.

Are HPLC autosamplers programmable for complex injection methods?

a.

Yes, modern HPLC autosamplers often come with software that allows users to program complex injection sequences, such as variable injection volumes or specific timing.

q.

What materials are used in the sample path of an HPLC autosampler?

a.

The sample path is often made of inert materials like stainless steel, PEEK, or PTFE to ensure chemical compatibility with a wide range of solvents and samples.