Explore a wide selection of new, used, and refurbished HPLC Columns available for purchase online. HPLC columns are critical for achieving accurate separations in high-performance liquid chromatography. Submit a quote request to connect with trusted vendors offering high-quality columns to suit your lab’s specific needs.
HPLC Columns
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Novasep ProChrom LC80.600.VE100 Dynamic Axial Compression Column
US
Excellent
Thermo Scientific Dionex ICS-5000+ System: SP-5, TC, AS-AP with Elemental Scientific SC-4DXX & FAST DX + Accessories
EU
Good
Timberline Mobile Phase Heater TL2500 HPLC FPLC GC CE Laboratory Equipment
US
Good
Agilent 1100 Series HPLC System with ColCom, Degasser, and Autosampler
UK
AS-IS
Question & Answers For:
HPLC Columns
Past Questions, Helpful Answers.
q.
What are HPLC columns used for?
a.
HPLC columns are used in high-performance liquid chromatography to separate, identify, and quantify components in a mixture.
q.
How do I choose the right HPLC column for my application?
a.
The choice depends on factors such as the nature of your sample, the separation mode (e.g., reversed-phase, normal phase), column dimensions, particle size, and the desired resolution.
q.
Can I use the same HPLC column for different types of samples?
a.
While some columns are versatile, it’s essential to check compatibility with the specific samples to avoid cross-contamination or reduced performance.
q.
How do I maintain and clean an HPLC column?
a.
Proper maintenance involves regular flushing with compatible solvents, avoiding extreme pH ranges, and storing the column as per the manufacturer's recommendations.
q.
What is the typical lifespan of an HPLC column?
a.
The lifespan varies based on usage, sample type, and maintenance but typically ranges from several months to a year under normal use.
q.
How can I ensure optimal performance of an HPLC column?
a.
Use high-purity solvents, filter samples before injection, operate within recommended pressure limits, and follow proper maintenance protocols.
q.
What is the significance of particle size in an HPLC column?
a.
Smaller particle sizes provide higher resolution but may require higher operating pressures. Choose the particle size based on your analytical needs and system capabilities.
q.
Can HPLC columns be reused?
a.
Yes, HPLC columns can be reused if properly maintained and not overloaded or exposed to incompatible samples or solvents.
q.
How do I determine if an HPLC column needs to be replaced?
a.
Signs include reduced resolution, increased backpressure, or inconsistent retention times, even after cleaning and troubleshooting.
q.
Are refurbished HPLC columns reliable?
a.
Refurbished columns can be a cost-effective option if sourced from trusted vendors and verified for quality and performance.